abbomax/Chk2/100ug/500-4914
市场价：
¥0.00
美元价：
0.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Chk2
Checkpoint kinase 2
Download Product PDFProduct Preparation
Purified recombinant CHK2 protein.
Background
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. Both Chk1 and Chk2 contain a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase,preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.In addition, CHKs interact with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in CHK2 gene have been linked with Li-Fraumeni syndrome, a highlypenetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclearprotein is a member of the CDS1subfamily of serine/threonine protein kinases. Three transcriptvariants (a, b, c) encoding different isoforms have been found.
PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping | SPECIFICITY This antibody recognizes ~70 kDa of human CHK2 protein. The other species are not tested. |
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer. | STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity.Avoid repeated freezing-thawing cycles. |
Gene ID
11200
Applications/Suggested Working Dilutions
Western Blot 0.1-1 µg/ml | ELISA 0.01-0.1 µg/ml | Immunoprecipitation 2-5µg/ml | IHC 2-10µg/ml | Flow cytometry 5-10µg/ml |
品牌介绍
Abbomax成立于2005年，是一家专业研发、生产、销售分子生物学试剂和提供生物科技CRO服务的高科技公司。产品主要包括抗体（一抗、二抗、磷酸化抗体）、蛋白多肽、脂质体、ELISA试剂盒、CellMax™ FFPE Cell Pellet Slide、TissueMax™ FFPE Cell Pellet Slide 等。这些产品受到了学术界的*好评。部分产品的详细列表：abbomax授权代理货号产品规格价格（美元）CellMax™ FFPE Cell Pellet Slide - A549T2102035$ 49.00CellMax™ FFPE Cell Pellet Slide - BT474T2101055$ 49.00CellMax™ FFPE Cell Pellet Slide - CaSkiT2103025$ 49.00CellMax™ FFPE Cell Pellet Slide - CHL-1T2106055$ 49.00
